Coding

Part:BBa_K2721020:Design

Designed by: Yunqian Li   Group: iGEM18_ZJU-China   (2018-09-26)


Spycatcher with histag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 55
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Considering that the tag-catcher pairs are ideal for binding and immobilization, our team use them as scaffold of protein assembly. With N-terminal His-tag, it can be purified by Ni-beads.

Source

SpyCatcher is derived from Streptococcus pyogenes fibronectin-binding protein.

References

1. Zakeri B, Fierer JO, Celik E, Chittock EC, Schwarz-Linek U, Moy VT, Howarth M. Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin. Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):E690-7.

2. P Hengen. Purification of His-Tag fusion proteins from Escherichia coli. Trends in Biochemical Sciences, 1995,20(7): 285-286.